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验证数据展示

  • WB analysis of Raji using 55379-1-AP

    WB analysis of Raji using 55379-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HLA-F Polyclonal antibody

HLA-F Polyclonal Antibody for WB, ELISA
Cat No. 55379-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, A431 cells, human placenta tissue, Jurkat cells, mouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55379-1-AP targets HLA-F in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name major histocompatibility complex, class I, F
Synonyms CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa, 66-70 kDa
GenBank Accession NumberNM_018950
Gene Symbol HLA-F
Gene ID (NCBI) 3134
RRIDAB_10863292
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30511
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

实验方案

Product Specific Protocols
WB protocol for HLA-F antibody 55379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols