验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 2 publications below |
产品信息
16913-1-AP targets HLA-G in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | HLA-G fusion protein Ag10577 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | major histocompatibility complex, class I, G |
| 别名 | HLA 6.0, HLA G, HLA G antigen, HLAG, HLA-G, MHC class I antigen G, MHC G |
| 计算分子量 | 338 aa, 38 kDa |
| 观测分子量 | 33-45 kDa |
| GenBank蛋白编号 | BC021708 |
| 基因名称 | HLA-G |
| Gene ID (NCBI) | 3135 |
| RRID | AB_2878330 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P17693 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). HLA-G is a non-classical MHC class I molecule with multiple immunoregulatory properties. HLA-G exhibits a restricted pattern of expression that includes placental extravillous trophoblasts at the maternal-fetal interface, where it abolishes maternal immune cell activity against fetus and establishes immune tolerance. Aberrant expression of HLA-G has been found in a variety of human neoplastic diseases. It plays an important role in the escape of tumor cells from immunosurveillance.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HLA-G antibody 16913-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging Cell Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts. | ||
Front Immunol Decreased USP2a Expression Inhibits Trophoblast Invasion and Associates With Recurrent Miscarriage. | ||
Cell Prolif Epiregulin promotes trophoblast epithelial-mesenchymal transition through poFUT1 and O-fucosylation by poFUT1 on uPA. |