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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 12870-1-AP (same clone as 12870-1-PBS)

    WB analysis using 12870-1-AP (same clone as 12870-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

    IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

    IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

    IHC staining of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

HLF Polyclonal antibody, PBS Only

HLF Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12870-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12870-1-PBS targets HLF in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HLF fusion protein Ag3533 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hepatic leukemia factor
别名
计算分子量 295 aa, 33 kDa
观测分子量 33kDa, 66 kDa
GenBank蛋白编号BC036093
基因名称 HLF
Gene ID (NCBI) 3131
RRIDAB_11182935
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16534
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hepatic leukemia factor (Hlf), is a novel hepatic protein of the proline and acidic amino acid-rich basic leucine zipper (bZip) family, previously studied for its role in circadian rhythm regulated neurotransmitter metabolism in the brain (PMID: 29166614). HLF is an essential regulator of HSC quiescence and that HLF deficiency leads to an exhaustion of HSCs in adult mouse BM during regeneration or following cytotoxic stress (PMID: 29262330).Hlf is able to bind DNA specifically as a homodimer or as a heterodimer with other PAR factors (PMID: 1516826). Western blot analysis detected bands  33 kDa whereas the 66 kDa could be the dimer form of Hlf.