验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
14336-1-AP targets HLX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | HLX fusion protein Ag5627 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | H2.0-like homeobox |
| 别名 | Homeobox protein HB24, HLX1, HB24, H2.0-like homeobox protein, H2.0 like homeobox protein |
| 计算分子量 | 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC033808 |
| 基因名称 | HLX |
| Gene ID (NCBI) | 3142 |
| RRID | AB_2232971 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14774 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In immune system, Hlx is a Th1-special transcription factor that interacts specifically with T-bet, and synergistically they promote IFN-γ expression when co-expressed. It can down-regulate the IL-4 receptor expression in naive CD4+ T cells [PMID:14688316]. Over expression of Hlx contributes ot the aberrant expression of IFN-γ in normal CD4+ T cells during the differentiation under Th2-polarizing conditions. In addition, Hlx is a negative regulator of IFN-γ production in NK cells and its inhibitory function is achieved at least in part through the proteasomal degradation of STAT4 [PMID:16424184].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HLX antibody 14336-1-AP | Download protocol |
| IHC protocol for HLX antibody 14336-1-AP | Download protocol |
| IF protocol for HLX antibody 14336-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |