验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse liver tissue, mouse kidney tissue, U2OS cells |
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
产品信息
20416-1-AP targets SPP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | SPP fusion protein Ag14158 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | histocompatibility (minor) 13 |
| 别名 | H13, hIMP1, histocompatibility (minor) 13, HM13, IMP 1, IMP1, IMPAS, IMPAS 1, Intramembrane protease 1, MSTP086, Presenilin like protein 3, PSENL3, PSL3, Signal peptide peptidase, SPP |
| 计算分子量 | 377 aa, 41 kDa |
| 观测分子量 | 45 kDa, 100 kDa |
| GenBank蛋白编号 | BC008938 |
| 基因名称 | HM13/SPP |
| Gene ID (NCBI) | 81502 |
| RRID | AB_10665421 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TCT9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPP antibody 20416-1-AP | Download protocol |
| IHC protocol for SPP antibody 20416-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Sci Topology surveillance of the lanosterol demethylase CYP51A1 by Signal Peptide Peptidase
| ||
PLoS One Visualizing active enzyme complexes using a photoreactive inhibitor for proximity ligation--application on γ-secretase. | ||
Cell Death Dis Histocompatibility Minor 13 (HM13), targeted by miR-760, exerts oncogenic role in breast cancer by suppressing autophagy and activating PI3K-AKT-mTOR pathway
| ||
Front Pharmacol Pan-cancer analysis suggests histocompatibility minor 13 is an unfavorable prognostic biomarker promoting cell proliferation, migration, and invasion in hepatocellular carcinoma
| ||
J Exp Clin Cancer Res miRNA-503 inhibition exerts anticancer effects and reduces tumor growth in mesothelioma |
