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验证数据展示

  • IHC staining of human colon using 18837-1-AP

    IHC staining of human colon using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 18837-1-AP

    IHC staining of human colon using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of mouse skin using 18837-1-AP

    WB analysis of mouse skin using 18837-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 18837-1-AP (HMCN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 18837-1-AP

    IF Staining of hTERT-RPE1 using 18837-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using HMCN1 antibody (18837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

HMCN1 Polyclonal antibody

HMCN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18837-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ARMD1, FBLN6, FIBL 6, FIBL6, Fibulin 6, hemicentin 1, HMCN1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skin tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18837-1-AP targets HMCN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemicentin 1
别名 ARMD1, FBLN6, FIBL 6, FIBL6, Fibulin 6, hemicentin 1, HMCN1
计算分子量 613 kDa, 600 kDa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号NM_031935
基因名称 HMCN1
Gene ID (NCBI) 83872
RRIDAB_2878563
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RW7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hemicentin-1 (HMCN1), also known as fibulin-6 (FIBL-6), is an extracellular protein of the immunoglobulin superfamily. HMCN1 consists of a von Willebrand A domain, tandem immunoglobulin modules, tandem EGF modules, and a fibulin carboxy-terminal module (PMID: 24912920). Mutations of HMCN1 gene may be associated with age-related macular degeneration. Three alternative transcripts encoding different HMCN1 isoforms have been described. This antibody raised against a synthesized peptide corresponding to 960-976 amino acids of human HMCN1 can recognize all three isoforms of HMCN1.

实验方案

Product Specific Protocols
WB protocol for HMCN1 antibody 18837-1-APDownload protocol
IHC protocol for HMCN1 antibody 18837-1-APDownload protocol
IF protocol for HMCN1 antibody 18837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Front Pharmacol

Artemisinin relieves myocardial ischemia-reperfusion injury via modulating miR-29b-3p and hemicentin 1

Authors - Junyu Han
View Article
mouseIHC

Front Cell Dev Biol

Hemicentin-1 is an essential extracellular matrix component during tooth root formation by promoting mesenchymal cells differentiation

Authors - Yujia Cui
View Article
mouseIHC

PLoS Pathog

Reduction of antisense transcription affects bovine leukemia virus replication and oncogenesis

Authors - Thomas Joris
View Article