验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12085-2-AP targets HMG20A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | HMG20A fusion protein Ag2718 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | high-mobility group 20A |
| 别名 | HMGX1, HMG domain-containing protein HMGX1, HMG domain-containing protein 1, HMG box-containing protein 20A, HMG box containing protein 20A |
| 计算分子量 | 347 aa, 40 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC021959 |
| 基因名称 | HMG20A |
| Gene ID (NCBI) | 10363 |
| RRID | AB_2117587 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NP66 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HMG20A antibody 12085-2-AP | Download protocol |
| IHC protocol for HMG20A antibody 12085-2-AP | Download protocol |
| IF protocol for HMG20A antibody 12085-2-AP | Download protocol |
| IP protocol for HMG20A antibody 12085-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs
| ||
Nat Commun KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics | ||
EMBO Mol Med LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma. | ||
Cancer Cell Int Circular RNA ABCB10 promotes hepatocellular carcinoma progression by increasing HMG20A expression by sponging miR-670-3p. | ||
Biochim Biophys Acta Interactome of the inhibitory isoform of the nuclear transporter Importin 13. | ||
Biochim Biophys Acta The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins. |
