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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29895-1-AP (same clone as 29895-1-PBS)

    WB analysis using 29895-1-AP (same clone as 29895-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29895-1-AP (HMGA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 29895-1-AP (same clone as 29895-1-PBS)

    IHC staining of human breast cancer using 29895-1-AP (same clone as 29895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 29895-1-AP (same clone as 29895-1-PBS)

    IHC staining of human prostate cancer using 29895-1-AP (same clone as 29895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 29895-1-AP (same clone as 29895-1-PBS)

    IHC staining of human prostate cancer using 29895-1-AP (same clone as 29895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 29895-1-AP (same clone as 29895-1-PBS)

    IHC staining of human pancreas cancer using 29895-1-AP (same clone as 29895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 29895-1-AP (same clone as 29895-1-PBS)

    IHC staining of human pancreas cancer using 29895-1-AP (same clone as 29895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 29895-1-AP (same clone as 29895-1-PBS)

    IF Staining of HUVEC using 29895-1-AP (same clone as 29895-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using HMGA1 antibody (29895-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

HMGA1 Polyclonal antibody, PBS Only

HMGA1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29895-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HMG-I(Y), HMGA1A, High mobility group protein R, High mobility group protein HMG-I/HMG-Y, High mobility group protein A1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

29895-1-PBS targets HMGA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HMGA1 fusion protein Ag31866 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 high mobility group AT-hook 1
别名 HMG-I(Y), HMGA1A, High mobility group protein R, High mobility group protein HMG-I/HMG-Y, High mobility group protein A1
观测分子量20 kDa
GenBank蛋白编号BC071864
基因名称 HMGA1
Gene ID (NCBI) 3159
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17096
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HMGA1, the high mobility group A1, encodes an oncofoetal protein that remodels the chromatin structure and promotes the interaction between transcriptional regulatory proteins and DNA in different cancers (PMID:8414980, PMID:11406267). The isoforms HMGA1a and HMGA1b can cross link DNA fibers in vitro and induce chromatin clustering in vivo (PMID:21596776). Mice lacking variant of HMGA1 are diabetic, show a cardiac hypertrophy and low levels of the insulin receptor (PMID:19664187). HMGA1 protein is mainly located in nucleus, especially in heterochromatin. HMGA1 is up-regulated in many tumours, including epithelial and mesenchymal tissue-originated tumours (PMID:30614613).