HMGB1 Monoclonal antibody, PBS Only

HMGB1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66525-1-PBS

产品说明书

CloneNo. 1D12A6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, Indirect ELISA

HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1, high mobility group box 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


Planning an IHC experiment? We recommend our IHCeasy HMGB1 Ready-To-Use IHC Kit. HMGB1 primary antibody included.

产品信息

66525-1-PBS targets HMGB1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HMGB1 fusion protein Ag1264 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 high-mobility group box 1
别名 HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1, high mobility group box 1
计算分子量 25 kDa
观测分子量 30 kDa
GenBank蛋白编号BC003378
基因名称 HMGB1
Gene ID (NCBI) 3146
RRIDAB_2881888
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09429
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.

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