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HMGB1 Recombinant antibody

HMGB1 Recombinant Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230182G6

Cat No : 82973-1-RR

Print datasheet

Synonyms

DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

验证数据展示

  • WB analysis using 82973-1-RR

    WB analysis using 82973-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82973-1-RR (HMGB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82973-1-RR

    IF Staining of HeLa using 82973-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HMGB1 antibody (82973-1-RR, Clone: 230182G6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). CoraLite®594-Phalloidin (red).

  • IHC staining of mouse brain using 82973-1-RR

    IHC staining of mouse brain using 82973-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82973-1-RR (HMGB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HMGB1 antibody included

经过测试的应用

Positive WB detected inL02 cells, Jurkat cells, HEK-293 cells, K-562 cells, HCT 116 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HMGB1 Ready-To-Use IHC Kit. HMGB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82973-1-RR targets HMGB1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen HMGB1 fusion protein Ag1264 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name high-mobility group box 1
Synonyms DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC003378
Gene Symbol HMGB1
Gene ID (NCBI) 3146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09429
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HMGB1 antibody 82973-1-RRDownload protocol
IHC protocol for HMGB1 antibody 82973-1-RRDownload protocol
IF protocol for HMGB1 antibody 82973-1-RRDownload protocol
FC protocol for HMGB1 antibody 82973-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols