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HMGB2 Monoclonal antibody
HMGB2 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1A7F10
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HEK-293 cells, MCF-7 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | human lung cancer tissue, mouse brain tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68185-1-Ig targets HMGB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | HMGB2 fusion protein Ag7989 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | high-mobility group box 2 |
Synonyms | HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2, high mobility group box 2 |
Calculated Molecular Weight | 24 kDa |
Observed Molecular Weight | 24-28 kDa |
GenBank Accession Number | BC000903 |
Gene Symbol | HMGB2 |
Gene ID (NCBI) | 3148 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P26583 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for HMGB2 antibody 68185-1-Ig | Download protocol |
IHC protocol for HMGB2 antibody 68185-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |