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HMGB2 Recombinant antibody, PBS Only

HMGB2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240453C11

Cat no : 83482-4-PBS

Print datasheet

Synonyms

HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2, high mobility group box 2

验证数据展示

  • WB analysis using 83482-4-RR (same clone as 83482-4-PBS)

    WB analysis using 83482-4-RR (same clone as 83482-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83482-4-RR (HMGB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83482-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83482-4-RR (same clone as 83482-4-PBS)

    FC experiment of HeLa using 83482-4-RR (same clone as 83482-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGB2 Recombinant antibody (83482-4-RR, Clone:240453C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83482-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83482-4-RR

    Affinity and Kinetic Characterization of 83482-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83482-4-RR against Human HMGB2 were performed. The affinity constant is 16.4 nM.

Planning an IHC experiment? We recommend our IHCeasy HMGB2 Ready-To-Use IHC Kit. HMGB2 primary antibody included.

产品信息

83482-4-PBS targets HMGB2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HMGB2 fusion protein Ag6135 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name high-mobility group box 2
Synonyms HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2, high mobility group box 2
Calculated Molecular Weight 24 kDa
GenBank Accession NumberBC001063
Gene Symbol HMGB2
Gene ID (NCBI) 3148
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP26583
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C