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HMGB2 Recombinant antibody, PBS Only

HMGB2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240453H5

Cat no : 83482-5-PBS

Print datasheet

Synonyms

HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2, high mobility group box 2

验证数据展示

  • IF Staining of HepG2 using 83482-5-RR (same clone as 83482-5-PBS)

    IF Staining of HepG2 using 83482-5-RR (same clone as 83482-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGB2 antibody (83482-5-RR, Clone: 240453H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83482-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83482-5-RR (same clone as 83482-5-PBS)

    FC experiment of HeLa using 83482-5-RR (same clone as 83482-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGB2 Recombinant antibody (83482-5-RR, Clone:240453H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83482-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83482-5-RR

    Affinity and Kinetic Characterization of 83482-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83482-5-RR against Human HMGB2 were performed. The affinity constant is 44.4 nM.

Planning an IHC experiment? We recommend our IHCeasy HMGB2 Ready-To-Use IHC Kit. HMGB2 primary antibody included.

产品信息

83482-5-PBS targets HMGB2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen HMGB2 fusion protein Ag6135 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name high-mobility group box 2
Synonyms HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2, high mobility group box 2
Calculated Molecular Weight 24 kDa
GenBank Accession NumberBC001063
Gene Symbol HMGB2
Gene ID (NCBI) 3148
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP26583
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C