验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue, mouse brain tissue, human heart tissue, rat liver tissue |
Positive IP detected in | mouse spleen tissue |
Positive IHC detected in | human liver tissue, human ovary tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16898-1-AP targets HMGCL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | HMGCL fusion protein Ag10426 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase |
别名 | HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4 |
计算分子量 | 325 aa, 34 kDa |
观测分子量 | 34 kDa |
GenBank蛋白编号 | BC010570 |
基因名称 | HMGCL |
Gene ID (NCBI) | 3155 |
RRID | AB_2295304 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P35914 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for HMGCL antibody 16898-1-AP | Download protocol |
IHC protocol for HMGCL antibody 16898-1-AP | Download protocol |
IP protocol for HMGCL antibody 16898-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma. | ||
Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. | ||
Hepatol Int HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility | ||
J Biol Chem Innate immune cell-intrinsic ketogenesis is dispensable for organismal metabolism and age-related inflammation | ||
Int J Biol Sci Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer
| ||
Cell Rep Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria |