HMGCL Polyclonal antibody

HMGCL Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 16898-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue, mouse brain tissue, human heart tissue, rat liver tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman liver tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16898-1-AP targets HMGCL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 HMGCL fusion protein Ag10426 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
别名 HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4
计算分子量 325 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC010570
基因名称 HMGCL
Gene ID (NCBI) 3155
RRIDAB_2295304
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35914
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HMGCL antibody 16898-1-APDownload protocol
IHC protocol for HMGCL antibody 16898-1-APDownload protocol
IP protocol for HMGCL antibody 16898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nat Cell Biol

BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.

Authors - Jin-Tao Li
humanWB

Cell Res

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang
humanWB,IHC

Hepatol Int

HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility

Authors - Xiaohan Cui
mouseWB

J Biol Chem

Innate immune cell-intrinsic ketogenesis is dispensable for organismal metabolism and age-related inflammation

Authors - Emily L Goldberg
human,mouseWB,IHC,IP

Int J Biol Sci

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer

Authors - Chenxi Zhong
  • KD Validated
mouseWB

Cell Rep

Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria

Authors - Sarah L Speck
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