• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HMGN2 Polyclonal antibody

HMGN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10953-1-AP

Print datasheet

Synonyms

Non-histone chromosomal protein HMG-17, HMG17, High mobility group nucleosome-binding domain-containing protein 2

验证数据展示

  • WB analysis of Jurkat using 10953-1-AP

    WB analysis of Jurkat using 10953-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10953-1-AP

    IHC staining of human prostate cancer using 10953-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 10953-1-AP

    IF Staining of HepG2 using 10953-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGN2 antibody (10953-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, HL-60 cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10953-1-AP targets HMGN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, ELISA
Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Immunogen HMGN2 fusion protein Ag1401 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name high-mobility group nucleosomal binding domain 2
Synonyms Non-histone chromosomal protein HMG-17, HMG17, High mobility group nucleosome-binding domain-containing protein 2
Calculated Molecular Weight 9 aa, 3 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC014644
Gene Symbol HMGN2
Gene ID (NCBI) 3151
RRIDAB_2118482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGN2 is a highly conserved nucleosomal protein which may involve in unfolding higher-order chromatin structure and facilitating the transcriptional activation of mammalian genes. It is the most abundant, ubiquitous and evolutionarily conserved nonhistione proteins found in the nuclei of higher eukaryotes. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer.

实验方案

Product Specific Protocols
WB protocol for HMGN2 antibody 10953-1-APDownload protocol
IHC protocol for HMGN2 antibody 10953-1-APDownload protocol
IF protocol for HMGN2 antibody 10953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance

Authors - Mengting Xu
View Article
humanELISA

Mol Cancer

HMGN2, a new anti-tumor effector molecule of CD8+ T cells.

Authors - Lin Su
View Article
pigWB

J Proteomics

DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase

Authors - Chen Zhou
View Article