验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, HL-60 cells, K-562 cells |
| Positive IHC detected in | human prostate cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| ELISA | See 1 publications below |
产品信息
10953-1-AP targets HMGN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | HMGN2 fusion protein Ag1401 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | high-mobility group nucleosomal binding domain 2 |
| 别名 | Non-histone chromosomal protein HMG-17, HMG17, High mobility group nucleosome-binding domain-containing protein 2 |
| 计算分子量 | 9 aa, 3 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC014644 |
| 基因名称 | HMGN2 |
| Gene ID (NCBI) | 3151 |
| RRID | AB_2118482 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05204 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HMGN2 is a highly conserved nucleosomal protein which may involve in unfolding higher-order chromatin structure and facilitating the transcriptional activation of mammalian genes. It is the most abundant, ubiquitous and evolutionarily conserved nonhistione proteins found in the nuclei of higher eukaryotes. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HMGN2 antibody 10953-1-AP | Download protocol |
| IHC protocol for HMGN2 antibody 10953-1-AP | Download protocol |
| IF protocol for HMGN2 antibody 10953-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance | ||
J Proteomics DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase | ||
Chem Sci A constitutional isomer selective chemical proteomic strategy for system-wide profiling of protein lysine 5-hydroxylation |
