• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HMGN4 Polyclonal antibody

HMGN4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11686-1-AP

Print datasheet

Synonyms

Non-histone chromosomal protein HMG-17-like 3, Non-histone chromosomal protein, NHC, HMG17L3, High mobility group nucleosome-binding domain-containing protein 4

验证数据展示

  • WB analysis of HeLa using 11686-1-AP

    WB analysis of HeLa using 11686-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11686-1-AP (HMGN4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 11686-1-AP

    IF Staining of HeLa using 11686-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11686-1-AP (HMGN4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11686-1-AP targets HMGN4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen HMGN4 fusion protein Ag2298 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name high mobility group nucleosomal binding domain 4
Synonyms Non-histone chromosomal protein HMG-17-like 3, Non-histone chromosomal protein, NHC, HMG17L3, High mobility group nucleosome-binding domain-containing protein 4
Calculated Molecular Weight 90 aa, 10 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC001282
Gene Symbol HMGN4
Gene ID (NCBI) 10473
RRIDAB_2118489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00479
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGN4, also named as High mobility group nucleosome-binding domain-containing protein 4, is a 90 amino acid protein, which belongs to the HMGN family. HMGN4 as a DNA-binding protein localizes in the nucleus. The calculated molecular weight of HMGN4 is 10 kDa, but the modified HMGN4 protein is about 15 kDa.

实验方案

Product Specific Protocols
WB protocol for HMGN4 antibody 11686-1-APDownload protocol
IF protocol for HMGN4 antibody 11686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols