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验证数据展示

  • WB analysis of HeLa using 11686-1-AP

    WB analysis of HeLa using 11686-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11686-1-AP (HMGN4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 11686-1-AP

    IF Staining of HeLa using 11686-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HMGN4 antibody (11686-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HMGN4 Polyclonal antibody

HMGN4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11686-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Non-histone chromosomal protein HMG-17-like 3, Non-histone chromosomal protein, NHC, HMG17L3, High mobility group nucleosome-binding domain-containing protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11686-1-AP targets HMGN4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HMGN4 fusion protein Ag2298 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 high mobility group nucleosomal binding domain 4
别名 Non-histone chromosomal protein HMG-17-like 3, Non-histone chromosomal protein, NHC, HMG17L3, High mobility group nucleosome-binding domain-containing protein 4
计算分子量 90 aa, 10 kDa
观测分子量 15 kDa
GenBank蛋白编号BC001282
基因名称 HMGN4
Gene ID (NCBI) 10473
RRIDAB_2118489
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00479
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGN4, also named as High mobility group nucleosome-binding domain-containing protein 4, is a 90 amino acid protein, which belongs to the HMGN family. HMGN4 as a DNA-binding protein localizes in the nucleus. The calculated molecular weight of HMGN4 is 10 kDa, but the modified HMGN4 protein is about 15 kDa.

实验方案

Product Specific Protocols
WB protocol for HMGN4 antibody 11686-1-APDownload protocol
IF protocol for HMGN4 antibody 11686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols