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HMGXB4 Polyclonal antibody

HMGXB4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 24821-1-AP

Print datasheet

Synonyms

HMG box containing protein 4, HMG box domain containing 4, HMG2L1, HMGBCG, HMGXB4, Protein HMGBCG, THC211630

验证数据展示

  • WB analysis of HeLa using 24821-1-AP

    WB analysis of HeLa using 24821-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24821-1-AP (HMGXB4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24821-1-AP

    IP experiment of HeLa using 24821-1-AP

    IP result of anti-HMGXB4 (IP:24821-1-AP, 4ug; Detection:24821-1-AP 1:500) with HeLa cells lysate 2560ug.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24821-1-AP targets HMGXB4 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen HMGXB4 fusion protein Ag20424 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HMG box domain containing 4
Synonyms HMG box containing protein 4, HMG box domain containing 4, HMG2L1, HMGBCG, HMGXB4, Protein HMGBCG, THC211630
Calculated Molecular Weight 601 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC104754
Gene Symbol HMGXB4
Gene ID (NCBI) 10042
RRIDAB_2879742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGXB4 also termed as HMG box containing protein 4 or HMG2L1 is a 601 amino acid protein, which contains one HMG box DNA-binding domain. HMGXB4 negatively regulates Wnt/beta-catenin signaling during development. HMG-2L1 is expressed in the nucleus and may play a role in transcriptional regulation.

实验方案

Product Specific Protocols
WB protocol for HMGXB4 antibody 24821-1-APDownload protocol
IP protocol for HMGXB4 antibody 24821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols