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HMP19 Polyclonal antibody

HMP19 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19423-1-AP

Print datasheet

Synonyms

HMP19, HMP19 protein, NSG2, Protein p19

验证数据展示

  • IHC staining of human hepatocirrhosis using 19423-1-AP

    IHC staining of human hepatocirrhosis using 19423-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19423-1-AP (HMP19 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 19423-1-AP

    IHC staining of human hepatocirrhosis using 19423-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19423-1-AP (HMP19 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 19423-1-AP

    IF Staining of HepG2 using 19423-1-AP

    Immunofluorescent analysis of HepG2 cells, using HMP19 antibody 19423-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 19423-1-AP is HMP19 Fusion Protein expressed in E. coli.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HMP19 fusion protein Ag13751 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HMP19 protein
Synonyms HMP19, HMP19 protein, NSG2, Protein p19
Calculated Molecular Weight 171 aa, 19 kDa
GenBank Accession NumberBC002619
Gene Symbol HMP19
Gene ID (NCBI) 51617
RRIDAB_10638444
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y328
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for HMP19 antibody 19423-1-APDownload protocol
IF protocol for HMP19 antibody 19423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Int J Cancer

Suppression of pancreatic cancer growth and metastasis by HMP19 identified through genome-wide shRNA screen.

Authors - Hiroshi Kurahara
View Article