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  • Featured Product
  • KD/KO Validated

HN1 Polyclonal antibody

HN1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14914-1-AP

Print datasheet

Synonyms

Androgen regulated protein 2, ARM2, HN1, HN1A

验证数据展示

  • WB analysis of HeLa using 14914-1-AP

    WB analysis of HeLa using 14914-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 14914-1-AP

    IHC staining of human ovary tumor using 14914-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with JPT1/HN1 antibody included
  • IHC staining of human ovary tumor using 14914-1-AP

    IHC staining of human ovary tumor using 14914-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with JPT1/HN1 antibody included
  • IP experiment of MCF-7 using 14914-1-AP

    IP experiment of MCF-7 using 14914-1-AP

    IP result of anti-HN1 (IP:14914-1-AP, 4ug; Detection:14914-1-AP 1:1000) with MCF-7 cells lysate 3200ug.

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, human testis tissue, HepG2 cells, A431 cells, LNCaP cells, mouse testis tissue, mouse colon tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy JPT1/HN1 Ready-To-Use IHC Kit. JPT1/HN1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14914-1-AP targets HN1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HN1 fusion protein Ag6712 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hematological and neurological expressed 1
Synonyms Androgen regulated protein 2, ARM2, HN1, HN1A
Calculated Molecular Weight 11 kDa, 16 kDa, 19 kDa
Observed Molecular Weight 23-30 kDa
GenBank Accession NumberBC001420
Gene Symbol HN1
Gene ID (NCBI) 51155
RRIDAB_10863118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HN1 (hematopoietic- and neurologic-expressed sequence 1) is a highly conserved protein that is expressed in developing and regenerating tissues. Overexpression of HN1 has been reported in various tumors like glioma and breast cancer. Hn1 has also been reported as a marker for human ovarian carcinoma and can distinguish epithelial ovarian carcinoma cells from normal ovarian surface epithelial cells. This collective information suggests that HN1 is involved in processes associated with cell proliferation, repair and/or growth.

实验方案

Product Specific Protocols
WB protocol for HN1 antibody 14914-1-APDownload protocol
IHC protocol for HN1 antibody 14914-1-APDownload protocol
IP protocol for HN1 antibody 14914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Cancer Lett

HN1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by interacting with STMN1.

Authors - Zongfu Pan
  • KO Validated
View Article
humanWB,IHC

Kaohsiung J Med Sci

Increased expression of hematological and neurological expressed 1 (HN1) is associated with a poor prognosis of hepatocellular carcinoma and its knockdown inhibits cell growth and migration partly by down-regulation of c-Met.

Authors - Jia-Jie Chen
  • KD Validated
View Article
humanWB

Chem Biol Drug Des

Matrine exerts an anti-tumor effect via regulating HN1 in triple breast cancer both in vitro and in vivo

Authors - Qiusheng Guo
View Article
humanWB,IF

Cancer Lett

Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer

Authors - Zongfu Pan
View Article
humanWB,IHC

Reprod Sci

Hematological and Neurological Expressed 1 Promotes Tumor Progression Through mTOR Signaling in Ovarian Cancer

Authors - Pin Li
  • KD Validated
View Article
humanWB,IHC,IF

Biochem Genet

Knocking Down HN1 Blocks Helicobacter pylori-Induced Malignant Phenotypes in Gastric Mucosal Cells and Inhibits Gastric Cancer Cell Proliferation, Cytoskeleton Remodeling, and Migration

Authors - Ying Huang
View Article