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验证数据展示

  • WB analysis using 16285-1-AP

    WB analysis using 16285-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16285-1-AP (HN1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 16285-1-AP

    IF Staining of A431 using 16285-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using HN1L antibody (16285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

HN1L Polyclonal antibody

HN1L Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16285-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Jupiter microtubule associated homolog 2, JPT2, HN1-like protein, Hematological and neurological expressed 1-like protein, C16orf34

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16285-1-AP targets HN1L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HN1L fusion protein Ag9400 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hematological and neurological expressed 1-like
别名 Jupiter microtubule associated homolog 2, JPT2, HN1-like protein, Hematological and neurological expressed 1-like protein, C16orf34
计算分子量 190 aa, 20 kDa
观测分子量20 kDa
GenBank蛋白编号BC014438
基因名称 HN1L
Gene ID (NCBI) 90861
RRIDAB_3085484
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H910
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hematological and neurological expressed 1-like protein (HN1L), also known as Jupiter microtubule-associated homolog 2 (JPT2), belongs to the jupiter family. HN1L is nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein required for NAADP-evoked intracellular calcium release (PubMed:33758061). HN1L enables NAADP to activate Ca2+ release from the endoplasmic reticulum through ryanodine receptors (PubMed:33758062). HN1L was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal systemHN1L is expressed in liver, kidney, prostate, testis and uterus (PMID: 15094197).

实验方案

Product Specific Protocols
WB protocol for HN1L antibody 16285-1-APDownload protocol
IF protocol for HN1L antibody 16285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols