验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue, SMMC-7721 cells, HuH-7 cells |
| Positive IP detected in | mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22426-1-AP targets HNF1A in WB, IHC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP, chIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | HNF1A fusion protein Ag18188 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HNF1 homeobox A |
| 别名 | Hepatocyte nuclear factor 1-alpha, HNF 1 alpha, HNF 1A, HNF1, HNF1 homeobox A |
| 计算分子量 | 631 aa, 67 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC104910 |
| 基因名称 | HNF1A |
| Gene ID (NCBI) | 6927 |
| RRID | AB_2879104 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P20823 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HNF-1 , a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF1 as a transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. It is required for the expression of several liver specific genes and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HNF1A antibody 22426-1-AP | Download protocol |
| IP protocol for HNF1A antibody 22426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer. | ||
Mol Ther Nucleic Acids The accessible promoter-mediated supplementary effect of host factors provides new insight into the tropism of SARS-CoV-2. | ||
Acta Pharmacol Sin Aloe-emodin exerts cholesterol-lowering effects by inhibiting proprotein convertase subtilisin/kexin type 9 in hyperlipidemic rats. | ||
Chem Biol Interact 6-Formylindolo(3,2-b)carbazole induced aryl hydrocarbon receptor activation prevents intestinal barrier dysfunction through regulation of claudin-2 expression.
| ||
J Exp Clin Cancer Res ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer. | ||
Cell Death Dis CBP/p300 HAT maintains the gene network critical for β cell identity and functional maturity. |
