验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, Jurkat cells, MDCK cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12533-1-AP targets HNF1B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | HNF1B fusion protein Ag3232 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HNF1 homeobox B |
| 别名 | TCF-2, TCF2, HNF-1-beta, HNF-1B, HNF 1 beta |
| 计算分子量 | 557 aa, 61 kDa |
| 观测分子量 | 58-65 kDa |
| GenBank蛋白编号 | BC017714 |
| 基因名称 | HNF1B |
| Gene ID (NCBI) | 6928 |
| RRID | AB_2116758 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35680 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. This is a rabbit polyclonal antibody raised against part chain of N-terminus of human HNF1B. HNF1B exists some isoforms with MV 61 kDa, 58 kDa, 46 kDa and 44 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HNF1B antibody 12533-1-AP | Download protocol |
| IHC protocol for HNF1B antibody 12533-1-AP | Download protocol |
| IF protocol for HNF1B antibody 12533-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids. | ||
Oncogene HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer.
| ||
Development Prox1 ablation in hepatic progenitors causes defective hepatocyte specification and increases biliary cell commitment. | ||
Sci Rep Complex bile duct network formation within liver decellularized extracellular matrix hydrogels. | ||
J Cell Mol Med HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer. | ||
Sci Rep ATM-deficiency increases genomic instability and metastatic potential in a mouse model of pancreatic cancer. |
