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HNMT Polyclonal antibody

HNMT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11874-1-AP

Print datasheet

Synonyms

HNMT S2, HNMT S1, HMT, Histamine N-methyltransferase, histamine N methyltransferase

验证数据展示

  • WB analysis using 11874-1-AP

    WB analysis using 11874-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11874-1-AP (HNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 11874-1-AP

    WB analysis of HepG2 using 11874-1-AP

    WB result of HNMT antibody (11874-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNMT transfected HepG2 cells.

  • IHC staining of human liver cancer using 11874-1-AP

    IHC staining of human liver cancer using 11874-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11874-1-AP (HNMT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 11874-1-AP

    IHC staining of human liver cancer using 11874-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11874-1-AP (HNMT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 11874-1-AP

    IF Staining of MCF-7 using 11874-1-AP

    Immunofluorescent analysis of MCF-7 cells using 11874-1-AP (HNMT antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHT-29 cells, human liver tissue, mouse liver tissue, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11874-1-AP targets HNMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HNMT fusion protein Ag2462 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histamine N-methyltransferase
Synonyms HNMT S2, HNMT S1, HMT, Histamine N-methyltransferase, histamine N methyltransferase
Calculated Molecular Weight 292 aa, 33 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC020677
Gene Symbol HNMT
Gene ID (NCBI) 3176
RRIDAB_2117048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50135
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.

实验方案

Product Specific Protocols
WB protocol for HNMT antibody 11874-1-APDownload protocol
IHC protocol for HNMT antibody 11874-1-APDownload protocol
IF protocol for HNMT antibody 11874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Glia

Molecular mechanism of histamine clearance by primary human astrocytes.

Authors - Yoshikawa Takeo T
View Article
humanWB

Cell Stem Cell

Glioblastoma stem cell-specific histamine secretion drives pro-angiogenic tumor microenvironment remodeling

Authors - Jiayi Chen
View Article