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HNRNPA1 Monoclonal antibody, PBS Only

HNRNPA1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5A4

Cat no : 67844-1-PBS

Print datasheet

Synonyms

hnRNP core protein A1, hnRNP A1, Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed, Helix-destabilizing protein, Helix destabilizing protein

验证数据展示

  • WB analysis using 67844-1-Ig (same clone as 67844-1-PBS)

    WB analysis using 67844-1-Ig (same clone as 67844-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67844-1-Ig (same clone as 67844-1-PBS)

    IHC staining of human lung cancer using 67844-1-Ig (same clone as 67844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPA1 antibody included
  • IHC staining of human lung cancer using 67844-1-Ig (same clone as 67844-1-PBS)

    IHC staining of human lung cancer using 67844-1-Ig (same clone as 67844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPA1 antibody included
  • IF Staining of HeLa using 67844-1-Ig (same clone as 67844-1-PBS)

    IF Staining of HeLa using 67844-1-Ig (same clone as 67844-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA1 antibody (67844-1-Ig, Clone: 1A5A4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67844-1-Ig (same clone as 67844-1-PBS)

    IHC staining of human breast cancer using 67844-1-Ig (same clone as 67844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67844-1-Ig (HNRNPA1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67844-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPA1 antibody included
Planning an IHC experiment? We recommend our IHCeasy HNRNPA1 Ready-To-Use IHC Kit. HNRNPA1 primary antibody included.

产品信息

67844-1-PBS targets HNRNPA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen HNRNPA1 fusion protein Ag17415 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein A1
Synonyms hnRNP core protein A1, hnRNP A1, Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed, Helix-destabilizing protein, Helix destabilizing protein
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC009600
Gene Symbol HNRNPA1
Gene ID (NCBI) 3178
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09651
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.