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验证数据展示

  • WB analysis using 31175-1-AP

    WB analysis using 31175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31175-1-AP (HNRNPAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 31175-1-AP

    IHC staining of mouse liver using 31175-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31175-1-AP (HNRNPAB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 31175-1-AP

    IF Staining of HeLa using 31175-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (31175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of Jurkat using 31175-1-AP

    IF Staining of Jurkat using 31175-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HNRNPAB antibody (31175-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

HNRNPAB Polyclonal antibody

HNRNPAB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31175-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

HNRPAB, hnRNP A/B, APOBEC1-binding protein 1, ABBP-1, ABBP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31175-1-AP targets HNRNPAB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HNRNPAB fusion protein Ag35010 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein A/B
别名 HNRPAB, hnRNP A/B, APOBEC1-binding protein 1, ABBP-1, ABBP1
计算分子量36 kDa
观测分子量36-40 kDa
GenBank蛋白编号BC036708
基因名称 HNRNPAB
Gene ID (NCBI) 3182
RRIDAB_3669882
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ99729
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).

实验方案

Product Specific Protocols
WB protocol for HNRNPAB antibody 31175-1-APDownload protocol
IHC protocol for HNRNPAB antibody 31175-1-APDownload protocol
IF protocol for HNRNPAB antibody 31175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols