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  • Featured Product
  • KD/KO Validated

HNRNPF Monoclonal antibody, PBS Only

HNRNPF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E7A4

Cat no : 67701-1-PBS

Print datasheet

Synonyms

hnRNP F, HNRNPF, HNRPF, mcs94 1, Nucleolin like protein mcs94 1, OK/SW cl.23

验证数据展示

  • WB analysis using 67701-1-Ig (same clone as 67701-1-PBS)

    WB analysis using 67701-1-Ig (same clone as 67701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67701-1-Ig (same clone as 67701-1-PBS)

    IF Staining of HepG2 using 67701-1-Ig (same clone as 67701-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPF antibody (67701-1-Ig, Clone: 2E7A4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

产品信息

67701-1-PBS targets HNRNPF in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen HNRNPF fusion protein Ag7128 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein F
Synonyms hnRNP F, HNRNPF, HNRPF, mcs94 1, Nucleolin like protein mcs94 1, OK/SW cl.23
Calculated Molecular Weight 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC001432
Gene Symbol HNRNPF
Gene ID (NCBI) 3185
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52597
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.