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  • Featured Product
  • KD/KO Validated

HNRNPF Monoclonal antibody, PBS Only

HNRNPF Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

2E7A4

Cat No : 67701-1-PBS

Print datasheet

Synonyms

hnRNP F, HNRNPF, HNRPF, mcs94 1, Nucleolin like protein mcs94 1, OK/SW cl.23

验证数据展示

  • WB analysis using 67701-1-Ig (same clone as 67701-1-PBS)

    WB analysis using 67701-1-Ig (same clone as 67701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67701-1-Ig (same clone as 67701-1-PBS)

    IF Staining of HepG2 using 67701-1-Ig (same clone as 67701-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPF antibody (67701-1-Ig, Clone: 2E7A4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

产品信息

67701-1-PBS targets HNRNPF in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen HNRNPF fusion protein Ag7128 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein F
Synonyms hnRNP F, HNRNPF, HNRPF, mcs94 1, Nucleolin like protein mcs94 1, OK/SW cl.23
Calculated Molecular Weight 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC001432
Gene Symbol HNRNPF
Gene ID (NCBI) 3185
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52597
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.