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验证数据展示

  • WB analysis using 67708-1-Ig

    WB analysis using 67708-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67708-1-Ig (HNRNPK antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human colon cancer using 67708-1-Ig

    IHC staining of human colon cancer using 67708-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HNRNPK antibody included
  • WB analysis using 67708-1-Ig

    WB analysis using 67708-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67708-1-Ig (HNRNPK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

HNRNPK Monoclonal antibody

HNRNPK Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67708-1-Ig

产品说明书

CloneNo. 1A10C8

宿主/亚型

Mouse / IgM

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

CSBP, FLJ41122, hnRNP K, HNRNPK, HNRPK, TUNP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HuH-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy HNRNPK Ready-To-Use IHC Kit. HNRNPK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67708-1-Ig targets HNRNPK in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 HNRNPK fusion protein Ag30093 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein K
别名 CSBP, FLJ41122, hnRNP K, HNRNPK, HNRPK, TUNP
计算分子量 463 aa, 51 kDa
观测分子量60 kDa
GenBank蛋白编号BC014980
基因名称 HNRNPK
Gene ID (NCBI) 3190
RRIDAB_2882899
偶联类型 Unconjugated
形式 Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDP61978
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability

实验方案

Product Specific Protocols
WB protocol for HNRNPK antibody 67708-1-IgDownload protocol
IHC protocol for HNRNPK antibody 67708-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols