验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, mouse liver tissue, PC-3 cells, RAW 264.7 cells |
| Positive IP detected in | BxPC-3 cells |
| Positive IHC detected in | human stomach cancer tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18354-1-AP targets HNRNPL in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, pig, bovine, hamster |
| 免疫原 | HNRNPL fusion protein Ag13211 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heterogeneous nuclear ribonucleoprotein L |
| 别名 | P/OKcl.14, HNRPL, hnRNP L |
| 计算分子量 | 572 aa, 62 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC069184 |
| 基因名称 | HNRNPL |
| Gene ID (NCBI) | 3191 |
| RRID | AB_2878537 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14866 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HNRNPL antibody 18354-1-AP | Download protocol |
| IHC protocol for HNRNPL antibody 18354-1-AP | Download protocol |
| IF protocol for HNRNPL antibody 18354-1-AP | Download protocol |
| IP protocol for HNRNPL antibody 18354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
RNA Biol CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation. | ||
J Proteome Res C1QBP Negatively Regulates the Activation of Oncoprotein YBX1 in the Renal Cell Carcinoma as Revealed by Interactomics Analysis. | ||
J Virol Heterogeneous Nuclear Ribonucleoprotein L Negatively Regulates Foot-and-Mouth Disease Virus Replication through Inhibition of Viral RNA Synthesis by Interacting with the Internal Ribosome Entry Site in the 5' Untranslated Region.
| ||
DNA Cell Biol HNRNPL Is Identified and Validated as a Prognostic Biomarker Associated with Microsatellite Instability in Human Gastric Cancer. |
