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验证数据展示

  • WB analysis using 82259-1-RR (same clone as 82259-1-PBS)

    WB analysis using 82259-1-RR (same clone as 82259-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82259-1-RR (HNRNPL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82259-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 82259-1-RR (same clone as 82259-1-PBS)

    IHC staining of human stomach cancer using 82259-1-RR (same clone as 82259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82259-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPL antibody included
  • IHC staining of human stomach cancer using 82259-1-RR (same clone as 82259-1-PBS)

    IHC staining of human stomach cancer using 82259-1-RR (same clone as 82259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82259-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPL antibody included
  • Affinity and Kinetic Characterization of 82259-1-RR

    Affinity and Kinetic Characterization of 82259-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82259-1-RR against Human HNRNPL were performed. The affinity constant is 6.54 nM.

HNRNPL Recombinant antibody, PBS Only

HNRNPL Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82259-1-PBS

产品说明书

CloneNo. 3J22

宿主/亚型

Rabbit / IgG

种属反应性

Human, Rat

应用

WB, IHC, Indirect ELISA

别名

P/OKcl.14, HNRPL, hnRNP L, 3J22

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HNRNPL Ready-To-Use IHC Kit. HNRNPL primary antibody included.

产品信息

82259-1-PBS targets HNRNPL in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Rat
免疫原 HNRNPL fusion protein Ag13211 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein L
别名 P/OKcl.14, HNRPL, hnRNP L, 3J22
计算分子量 572 aa, 62 kDa
观测分子量64 kDa
GenBank蛋白编号BC069184
基因名称 HNRNPL
Gene ID (NCBI) 3191
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14866
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.