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HNRNPR Polyclonal antibody

HNRNPR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 29980-1-AP

Print datasheet

Synonyms

FLJ25714, hnRNP R, HNRNPR, HNRPR

验证数据展示

  • WB analysis using 29980-1-AP

    WB analysis using 29980-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29980-1-AP (HNRNPR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29980-1-AP

    IHC staining of mouse brain using 29980-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29980-1-AP

    IHC staining of mouse brain using 29980-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29980-1-AP

    IHC staining of mouse brain using 29980-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29980-1-AP (HNRNPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 29980-1-AP

    IF Staining of HepG2 using 29980-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HNRNPR antibody (29980-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29980-1-AP targets HNRNPR in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, RIP
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen HNRNPR fusion protein Ag30339 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein R
Synonyms FLJ25714, hnRNP R, HNRNPR, HNRPR
Calculated Molecular Weight 71 kDa
Observed Molecular Weight65-82 kDa
GenBank Accession NumberBC001449
Gene Symbol HNRNPR
Gene ID (NCBI) 10236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43390
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)

实验方案

Product Specific Protocols
WB protocol for HNRNPR antibody 29980-1-APDownload protocol
IHC protocol for HNRNPR antibody 29980-1-APDownload protocol
IF protocol for HNRNPR antibody 29980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Genet

HnRNPR strongly represses splicing of a critical exon associated with spinal muscular atrophy through binding to an exonic AU-rich element

Authors - Tao Jiang
View Article
humanWB,RIP

Biochim Biophys Acta Mol Basis Dis

HNRNPA2B1 and HNRNPR stabilize ASCL1 in an m6A-dependent manner to promote neuroblastoma progression

Authors - Ting Hu
View Article