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  • Featured Product
  • KD/KO Validated

HNRNPU Polyclonal antibody, PBS Only

HNRNPU Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16365-1-PBS

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Synonyms

验证数据展示

  • WB analysis of HEK-293 using 16365-1-AP (same clone as 16365-1-PBS)

    WB analysis of HEK-293 using 16365-1-AP (same clone as 16365-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16365-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 16365-1-AP (same clone as 16365-1-PBS)

    IP experiment of HeLa using 16365-1-AP (same clone as 16365-1-PBS)

    IP result of anti-HNRNPU (IP:16365-1-AP, 4ug; Detection:16365-1-AP 1:8000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 16365-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 16365-1-AP (same clone as 16365-1-PBS)

    WB analysis of HeLa using 16365-1-AP (same clone as 16365-1-PBS)

    WB result of HNRNPU antibody (16365-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPU transfected HeLa cells. This data was developed using the same antibody clone with 16365-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 16365-1-AP (same clone as 16365-1-PBS)

    IHC staining of mouse kidney using 16365-1-AP (same clone as 16365-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16365-1-AP (HNRNPU antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16365-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPU antibody included
  • IF Staining of HepG2 using 16365-1-AP (same clone as 16365-1-PBS)

    IF Staining of HepG2 using 16365-1-AP (same clone as 16365-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPU antibody (16365-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16365-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 16365-1-AP (same clone as 16365-1-PBS)

    FC experiment of HepG2 using 16365-1-AP (same clone as 16365-1-PBS)

    1X10^6 HepG2 cells were stained with 0.2ug HNRNPU antibody (16365-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. This data was developed using the same antibody clone with 16365-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HNRNPU Ready-To-Use IHC Kit. HNRNPU primary antibody included.

产品信息

16365-1-PBS targets HNRNPU in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HNRNPU fusion protein Ag9151 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Synonyms
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC003367
Gene Symbol HNRNPU
Gene ID (NCBI) 3192
RRIDAB_10596481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00839
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).