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HNRPDL Polyclonal antibody

HNRPDL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10660-2-AP

Print datasheet

Synonyms

hnRNP D-like, hnRNP DL, hnRNP D like, HNRNP, Heterogeneous nuclear ribonucleoprotein D-like

验证数据展示

  • WB analysis using 10660-2-AP

    WB analysis using 10660-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10660-2-AP (HNRPDL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 10660-2-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 10660-2-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10660-2-AP (HNRPDL antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10660-2-AP targets HNRPDL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HNRPDL fusion protein Ag1046 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein D-like
Synonyms hnRNP D-like, hnRNP DL, hnRNP D like, HNRNP, Heterogeneous nuclear ribonucleoprotein D-like
Calculated Molecular Weight 46 aa, 4 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC007392
Gene Symbol HNRPDL
Gene ID (NCBI) 9987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14979
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HNRPDL antibody 10660-2-APDownload protocol
IHC protocol for HNRPDL antibody 10660-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Biochem Funct

Overexpression of JKTBP1 induces androgen-independent LNCaP cell proliferation through activation of epidermal growth factor-receptor (EGF-R).

Authors - Yong-Yang Wu
View Article