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  • Featured Product
  • KD/KO Validated

HNRPLL Polyclonal antibody

HNRPLL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26769-1-AP

Print datasheet

Synonyms

Stromal RNA-regulating factor, SRRF, hnRNPLL, Heterogeneous nuclear ribonucleoprotein L-like

验证数据展示

  • WB analysis using 26769-1-AP

    WB analysis using 26769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26769-1-AP (HNRPLL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 26769-1-AP

    IHC staining of mouse kidney using 26769-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 26769-1-AP

    IF Staining of HepG2 using 26769-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRPLL antibody (26769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inmouse kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26769-1-AP targets HNRPLL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen HNRPLL fusion protein Ag25287 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein L-like
Synonyms Stromal RNA-regulating factor, SRRF, hnRNPLL, Heterogeneous nuclear ribonucleoprotein L-like
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC017480
Gene Symbol HNRPLL
Gene ID (NCBI) 92906
RRIDAB_2880628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVV9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNRPLL, also named as HNRNPLL and SRRF, is an RNA binding protein that regulates alternative splicing of pre-mRNA. HnRNPLL is up-regulated in stimulated T cells, bound CD45 transcripts, and is a key inducible regulator of CD45 alternative splicing (PMID: 18669861). HNRPLL has 5 isoforms with the molecular weights of 31-60 kDa. Additionally, studies have shown that HNRPLL is a metastasis suppressor gene in colorectal cancer (PMID: 28360095).

实验方案

Product Specific Protocols
WB protocol for HNRPLL antibody 26769-1-APDownload protocol
IHC protocol for HNRPLL antibody 26769-1-APDownload protocol
IF protocol for HNRPLL antibody 26769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Stem Cell Rev Rep

Lnc-PPP2R1B Mediates the Alternative Splicing of PPP2R1B by Interacting and Stabilizing HNRNPLL and Promotes Osteogenesis of MSCs

Authors - Shuping Peng
View Article
humanWB,IHC,IF

J Exp Clin Cancer Res

Energy stress-induced circZFR enhances oxidative phosphorylation in lung adenocarcinoma via regulating alternative splicing

Authors - Zhifei Ma
  • KD Validated
View Article