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  • Featured Product
  • KD/KO Validated

HO-1 Recombinant antibody, PBS Only

HO-1 Recombinant Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2M6

Cat No : 81281-1-PBS

Print datasheet

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HSP32

验证数据展示

  • WB analysis using 81281-1-RR (same clone as 81281-1-PBS)

    WB analysis using 81281-1-RR (same clone as 81281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81281-1-RR (HO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

    WB analysis of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

    WB result of HO-1 antibody (81281-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1 transfected HeLa cells. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

    IP experiment of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

    IP result of anti-HO-1 (IP:81281-1-RR, 4ug; Detection:81281-1-RR 1:8000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

  • Affinity experiments of 81281-1-RR

    Affinity experiments of 81281-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81281-1-RR against Human HO-1 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy HO-1/HMOX1 Ready-To-Use IHC Kit. HO-1/HMOX1 primary antibody included.

产品信息

81281-1-PBS targets HO-1 in WB, IP, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IP, Indirect ELISA Application Description
Tested Reactivity Human, Rat
Immunogen HO-1 fusion protein Ag1190 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heme oxygenase (decycling) 1
Synonyms bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HSP32
Calculated Molecular Weight 33 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC001491
Gene Symbol HMOX1
Gene ID (NCBI) 3162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09601
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).