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验证数据展示

  • WB analysis using 81281-1-RR (same clone as 81281-1-PBS)

    WB analysis using 81281-1-RR (same clone as 81281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81281-1-RR (HO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

    WB analysis of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

    WB result of HO-1 antibody (81281-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1 transfected HeLa cells. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

    IP experiment of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

    IP result of anti-HO-1 (IP:81281-1-RR, 4ug; Detection:81281-1-RR 1:8000) with HeLa cells lysate 1120 ug. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81281-1-RR

    Affinity and Kinetic Characterization of 81281-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81281-1-RR against Human HO-1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

HO-1 Recombinant antibody, PBS Only

HO-1 Uni-rAbTM Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 81281-1-PBS

产品说明书

CloneNo. 2M6

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IP, Indirect ELISA

别名

HMOX1, HO-1/HMOX1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HO-1/HMOX1 Ready-To-Use IHC Kit. HO-1/HMOX1 primary antibody included.

产品信息

81281-1-PBS targets HO-1 in WB, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 HO-1 fusion protein Ag1190 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heme oxygenase (decycling) 1
别名 HMOX1, HO-1/HMOX1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1
计算分子量 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC001491
基因名称 HO-1
Gene ID (NCBI) 3162
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09601
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).