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HOGA1 Polyclonal antibody

HOGA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 27755-1-AP

Print datasheet

Synonyms

C10orf65, DHDPS like protein, DHDPSL, HOGA1, Protein 569272

验证数据展示

  • WB analysis using 27755-1-AP

    WB analysis using 27755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27755-1-AP (HOGA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27755-1-AP targets HOGA1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen HOGA1 fusion protein Ag26849 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 10 open reading frame 65
Synonyms C10orf65, DHDPS like protein, DHDPSL, HOGA1, Protein 569272
Observed Molecular Weight35 kDa
GenBank Accession NumberBC045550
Gene Symbol C10orf65
Gene ID (NCBI) 112817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86XE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HOGA1 (4-hydroxy-2-oxoglutarate aldolase), also known as C10orf65, DHDPSL, is a mitochondrial enzyme that plays a gatekeeper role in hydroxyproline metabolis. HOGA1 is primarily expressed in liver and kidney and catalyzes the final step of mitochondrial hydroxyproline metabolism\ from 4-hydroxy-2-oxoglutarate to glyoxylate and pyruvate (PMID: 31696211). Identification of mutations in the HOGA1 gene as the cause of autosomal recessive primary hyperoxaluria (PH) type III has revitalized research in the field of PH and related stone disease (PMID: 22781098).

实验方案

Product Specific Protocols
WB protocol for HOGA1 antibody 27755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols