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验证数据展示

  • WB analysis of LNCaP using 67377-1-Ig

    WB analysis of LNCaP using 67377-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67377-1-Ig (HOMER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 67377-1-Ig

    IHC staining of human endometrial cancer using 67377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67377-1-Ig (HOMER2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 67377-1-Ig

    IHC staining of human endometrial cancer using 67377-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67377-1-Ig (HOMER2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HOMER2 Monoclonal antibody

HOMER2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67377-1-Ig

Print datasheet

CloneNo. 1G5G1

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

ACPD, CPD, Cupidin, HOMER 2, homer homolog 2 (Drosophila), Homer protein homolog 2, HOMER2, HOMER2A, HOMER2B, Vesl 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67377-1-Ig targets HOMER2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen HOMER2 fusion protein Ag25177 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name homer homolog 2 (Drosophila)
Synonyms ACPD, CPD, Cupidin, HOMER 2, homer homolog 2 (Drosophila), Homer protein homolog 2, HOMER2, HOMER2A, HOMER2B, Vesl 2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC012109
Gene Symbol HOMER2
Gene ID (NCBI) 9455
RRIDAB_2882624
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NSB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HOMER2 antibody 67377-1-IgDownload protocol
IHC protocol for HOMER2 antibody 67377-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols