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验证数据展示

  • WB analysis using 23965-1-AP

    WB analysis using 23965-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23965-1-AP (HOMEZ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23965-1-AP

    IHC staining of human testis using 23965-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23965-1-AP (HOMEZ Antibody) at dilution of 1:50.

  • IF Staining of HEK-293 using 23965-1-AP

    IF Staining of HEK-293 using 23965-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using HOMEZ antibody (23965-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

HOMEZ Polyclonal antibody

HOMEZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23965-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

HOMEZ, KIAA1443

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23965-1-AP targets HOMEZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 HOMEZ fusion protein Ag21090 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homeobox and leucine zipper encoding
别名 HOMEZ, KIAA1443
计算分子量 525 aa, 59 kDa
观测分子量 65 kDa
GenBank蛋白编号BC130392
基因名称 HOMEZ
Gene ID (NCBI) 57594
RRIDAB_2879378
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8IX15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HOMEZ antibody 23965-1-APDownload protocol
IHC protocol for HOMEZ antibody 23965-1-APDownload protocol
IF protocol for HOMEZ antibody 23965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Transl Androl Urol

Homeodomain protein HOMEZ is dispensable for male fertility in mice.

Authors - Qiuling Yue
View Article