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验证数据展示

  • WB analysis using 23965-1-AP

    WB analysis using 23965-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23965-1-AP (HOMEZ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23965-1-AP

    IHC staining of human testis using 23965-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23965-1-AP (HOMEZ Antibody) at dilution of 1:50.

  • IF Staining of HEK-293 using 23965-1-AP

    IF Staining of HEK-293 using 23965-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using HOMEZ antibody (23965-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

HOMEZ Polyclonal antibody

HOMEZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23965-1-AP

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Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HOMEZ, KIAA1443

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23965-1-AP targets HOMEZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen HOMEZ fusion protein Ag21090 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name homeobox and leucine zipper encoding
Synonyms HOMEZ, KIAA1443
Calculated Molecular Weight 525 aa, 59 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC130392
Gene Symbol HOMEZ
Gene ID (NCBI) 57594
RRIDAB_2879378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8IX15
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HOMEZ antibody 23965-1-APDownload protocol
IHC protocol for HOMEZ antibody 23965-1-APDownload protocol
IF protocol for HOMEZ antibody 23965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Transl Androl Urol

Homeodomain protein HOMEZ is dispensable for male fertility in mice.

Authors - Qiuling Yue
View Article