HORMAD1 Polyclonal antibody
HORMAD1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13917-1-AP targets HORMAD1 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | HORMAD1 fusion protein Ag4914 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | HORMA domain containing 1 |
| Synonyms | NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC047406 |
| Gene Symbol | HORMAD1 |
| Gene ID (NCBI) | 84072 |
| RRID | AB_2120844 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86X24 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734]. HORMAD1 exists as various isoform and the range of molecular weight of isoforms are sbout 36-45 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HORMAD1 antibody 13917-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination. | ||
Nucleic Acids Res NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells. | ||
Curr Biol Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes. | ||
PLoS Genet Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis. | ||
PLoS Genet Meiotic cohesins mediate initial loading of HORMAD1 to the chromosomes and coordinate SC formation during meiotic prophase. |