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验证数据展示

  • WB analysis using 28719-1-AP

    WB analysis using 28719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28719-1-AP (HORMAD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28719-1-AP

    IHC staining of human breast cancer using 28719-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28719-1-AP (HORMAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 28719-1-AP

    IHC staining of mouse skin using 28719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28719-1-AP (HORMAD1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 28719-1-AP

    IHC staining of mouse skin using 28719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28719-1-AP (HORMAD1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skin using 28719-1-AP

    IHC staining of rat skin using 28719-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28719-1-AP (HORMAD1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skin using 28719-1-AP

    IHC staining of rat skin using 28719-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28719-1-AP (HORMAD1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HORMAD1 Polyclonal antibody

HORMAD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28719-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat testis tissue, mouse testis tissue
Positive IHC detected inhuman breast cancer tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28719-1-AP targets HORMAD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen HORMAD1 fusion protein Ag29859 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HORMA domain containing 1
Synonyms NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC047406
Gene Symbol HORMAD1
Gene ID (NCBI) 84072
RRIDAB_2881199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86X24
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They are also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734]. HORMAD1 exists as various isoform and the range of molecular weight of isoforms are about 36-45 kDa.

实验方案

Product Specific Protocols
WB protocol for HORMAD1 antibody 28719-1-APDownload protocol
IHC protocol for HORMAD1 antibody 28719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC,WB

Sci Rep

A maternal high-fat diet induces fetal origins of NASH-HCC in mice.

Authors - Takao Takiyama
View Article