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HOXA9 Polyclonal antibody

HOXA9 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 29978-1-AP

Print datasheet

Synonyms

HOX1G, HOX1.7, HOX1, Homeobox protein Hox-A9, Homeobox protein Hox-1G

验证数据展示

  • IP experiment of HEK-293 using 29978-1-AP

    IP experiment of HEK-293 using 29978-1-AP

    IP result of anti-HOXA9 (IP:29978-1-AP, 4ug; Detection:29978-1-AP 1:800) with HEK-293 cells lysate 1480 ug.

  • IHC staining of human colon using 29978-1-AP

    IHC staining of human colon using 29978-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29978-1-AP (HOXA9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29978-1-AP targets HOXA9 in IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, ELISA Application Description
Tested Reactivity human
Immunogen HOXA9 fusion protein Ag32309 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name homeobox A9
Synonyms HOX1G, HOX1.7, HOX1, Homeobox protein Hox-A9, Homeobox protein Hox-1G
Calculated Molecular Weight 30 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberBC010023
Gene Symbol HOXA9
Gene ID (NCBI) 3205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31269
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homeobox protein Hox-A9 (HOXA9) also known as HOX1G,a member of HOX family belonging to the HOXA cluster, is often studied in acute myeloid leukemia (AML), which is linked to proliferation, differentiation, and progenitor self-renewal maintenance. It is located in nucleoplasm and functions as a critical regulator of hematopoiesis, essential for the maintenance of hematopoietic stem cells and their differentiation into myeloid lineages (PMID: 34743404). Many post-transcriptional events including microRNAs, long non-coding RNAs, and epigenetic modification could also determine HOXA9 protein level and function (PMID: 36376832).

实验方案

Product Specific Protocols
IHC protocol for HOXA9 antibody 29978-1-APDownload protocol
IP protocol for HOXA9 antibody 29978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols