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验证数据展示

  • WB analysis using 26384-1-AP

    WB analysis using 26384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26384-1-AP (HOXB13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 26384-1-AP

    IHC staining of human prostate cancer using 26384-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26384-1-AP (HOXB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 26384-1-AP

    IHC staining of human prostate cancer using 26384-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26384-1-AP (HOXB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 26384-1-AP

    IF Staining of PC-3 using 26384-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using HOXB13 antibody (26384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

HOXB13 Polyclonal antibody

HOXB13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26384-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

homeobox B13, Homeobox protein Hox B13, HOXB13, PSGD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26384-1-AP targets HOXB13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen HOXB13 fusion protein Ag24757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name homeobox B13
Synonyms homeobox B13, Homeobox protein Hox B13, HOXB13, PSGD
Calculated Molecular Weight 284 aa, 31 kDa
Observed Molecular Weight 31-35 kDa
GenBank Accession NumberBC007092
Gene Symbol HOXB13
Gene ID (NCBI) 10481
RRIDAB_3085864
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92826
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The homeobox (HOX) is a specific and widely distributed DNA sequence characterizing genes that are responsive to HOX transcription factors. Homeobox B13 (HOXB13) is one of the numerous DNA-binding transcription factors of the HOX gene family that has been implicated in the development of normal prostate and cancer (PMID: 25825985). In addition, high-level expression of HOXB13 is closely associated with tumor angiogenesis and poor prognosis of hepatocellular carcinoma(PMID: 25031711).

实验方案

Product Specific Protocols
WB protocol for HOXB13 antibody 26384-1-APDownload protocol
IHC protocol for HOXB13 antibody 26384-1-APDownload protocol
IF protocol for HOXB13 antibody 26384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols