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HOXB9 Recombinant antibody

HOXB9 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241866C6

Cat no : 84609-3-RR

Print datasheet

Synonyms

HOX2E, HOX2.5, Homeobox protein Hox-B9, Homeobox protein Hox-2E, Homeobox protein Hox-2.5

验证数据展示

  • WB analysis of K-562 using 84609-3-RR

    WB analysis of K-562 using 84609-3-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 84609-3-RR (HOXB9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84609-3-RR

    WB analysis using 84609-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84609-3-RR (HOXB9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84609-3-RR

    Affinity and Kinetic Characterization of 84609-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84609-3-RR against Human HOXB9 were performed. The affinity constant is 40.5 nM.

经过测试的应用

Positive WB detected inK-562 cells, HCT 116 cells, HT-29 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84609-3-RR targets HOXB9 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen HOXB9 fusion protein Ag34837 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name homeobox B9
Synonyms HOX2E, HOX2.5, Homeobox protein Hox-B9, Homeobox protein Hox-2E, Homeobox protein Hox-2.5
Calculated Molecular Weight 250 aa, 28 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC015565
Gene Symbol HOXB9
Gene ID (NCBI) 3219
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17482
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HOXB9 is a member of the Homeobox (HOX) gene family, which plays a crucial role in embryonic development and is involved in the regulation of gene expression during this process. In addition to its role in embryogenesis, HOXB9 has been implicated in various cancers, including breast and prostate cancer. It is overexpressed in breast cancer tissue and is regulated by estrogen, playing a critical role in angiogenesis. HOXB9 overexpression has been shown to stimulate three-dimensional colony formation in soft-agar assays, indicating its role in tumorigenesis. Furthermore, HOXB9 binds to the promoters of various tumor growth and angiogenic factors, regulating their expression, and its homeodomain plays a crucial role in transcriptional regulation and tumorigenesis.

实验方案

Product Specific Protocols
WB protocol for HOXB9 antibody 84609-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols