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HOXC13 Polyclonal antibody

HOXC13 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27002-1-AP

Print datasheet

Synonyms

HOX3G

验证数据展示

  • WB analysis using 27002-1-AP

    WB analysis using 27002-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27002-1-AP (HOXC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 27002-1-AP

    IF Staining of HeLa using 27002-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HOXC13 antibody (27002-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHeLa cells, NCCIT cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27002-1-AP targets HOXC13 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen HOXC13 fusion protein Ag25544 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name homeobox C13
Synonyms HOX3G
Calculated Molecular Weight35 kDa, 330 aa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC090850
Gene Symbol HOXC13
Gene ID (NCBI) 3229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31276
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HOXC13 is a member of HOX proteins. The HOX family includes 39 paralogous proteins expressed from the HOXA, HOXB, HOXC, and HOXD gene clusters, and these function early in embryonic development to establish cell and tissue identity and to regulate cell proliferation, differentiation, and survival (PMID: 30974835). Human HOXC13, which is expressed in the hair follicle and nail matrix, is involved in the regulation of keratin gene expression (PMID: 11714694). Mutations in human HOXC13 cause ectodermal dysplasia 9, a severe defect of hair and nails (PMID: 23063621).

实验方案

Product Specific Protocols
WB protocol for HOXC13 antibody 27002-1-APDownload protocol
IF protocol for HOXC13 antibody 27002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols