HOXD13 Polyclonal antibody
HOXD13 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, rat brain tissue, Jurkat cells, mouse lung tissue, mouse brain tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human brain tissue, human hepatocirrhosis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
18736-1-AP targets HOXD13 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | homeobox D13 |
| Synonyms | BDE, BDSD, homeobox D13, Homeobox protein Hox 4I, Homeobox protein Hox D13, HOX4I, HOXD13, SPD |
| Calculated Molecular Weight | 343 aa, 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC148863 |
| Gene Symbol | HOXD13 |
| Gene ID (NCBI) | 3239 |
| RRID | AB_2119926 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35453 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HOXD13, also named as HOX4I, belongs to the Abd-B homeobox family. HOXD13 is a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Mutation of HOXD13 will cause synpolydactyly (SPD), brachydactyly type D (BDD), syndactyly type 5brachydactyly-syndactyly syndrome (BDSD) or brachydactyly type E (BDE).Present polyclonal anti-HOX13D antibody(18736-1-AP) is produced by immunizing animals with part of N-terminus chain of HOX13D and detect a 36-kDa band in cell and tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HOXD13 antibody 18736-1-AP | Download protocol |
| IHC protocol for HOXD13 antibody 18736-1-AP | Download protocol |
| IP protocol for HOXD13 antibody 18736-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Pharm Sialic Acid-Functionalized PEG-PLGA Microspheres Loading Mitochondrial Targeting Modified Curcumin for Acute Lung Injury Therapy. | ||
Cell Death Dis A novel Hoxd13 mutation causes synpolydactyly and promotes osteoclast differentiation by regulating pSmad5/p65/c-Fos/Rank axis |
