Haptoglobin Monoclonal antibody

Haptoglobin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B4B9

Cat no : 66229-1-Ig

Print datasheet

Synonyms

BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S, Zonulin



经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HP Ready-To-Use IHC Kit. HP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66229-1-Ig targets Haptoglobin in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, pig
Cited Reactivitymonkey
Immunogen Haptoglobin fusion protein Ag9927 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name haptoglobin
Synonyms BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S, Zonulin
Calculated Molecular Weight281aa,31 kDa; 228aa,25 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC058031
Gene Symbol HP
Gene ID (NCBI) 3240
RRIDAB_2881620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00738
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn’s disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).

实验方案

Product Specific Protocols
WB protocol for Haptoglobin antibody 66229-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
monkeyWB

Int J Radiat Oncol Biol Phys

Identification of Novel Biomarkers for Acute Radiation Injury Using Multiomics Approach and Nonhuman Primate Model

Authors - Amrita K Cheema