验证数据展示
经过测试的应用
| Positive WB detected in | mouse thymus tissue, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
24556-1-AP targets HP1BP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | HP1BP3 fusion protein Ag18709 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heterochromatin protein 1, binding protein 3 |
| 别名 | Protein HP1-BP74, Protein HP1 BP74, HP1 BP74, Heterochromatin protein 1-binding protein 3 |
| 计算分子量 | 553 aa, 61 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC045660 |
| 基因名称 | HP1BP3 |
| Gene ID (NCBI) | 50809 |
| RRID | AB_2879606 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5SSJ5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Heterochromatin protein 1 binding protein 3(HP1BP3) associates with chromatin throughout the cell cycle and is required to maintain higher order chromatin structure during metaphase. HP1BP3 as a novel modulator of cognitive aging using a genetically diverse population of mice and confirm that HP1BP3 protein levels are significantly reduced in the hippocampi of cognitively impaired elderly humans relative to cognitively intact controls. Double-band staining is typical of HP1BP3 expression, indicative of multiple splice variants present in brain tissue.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HP1BP3 antibody 24556-1-AP | Download protocol |
| IHC protocol for HP1BP3 antibody 24556-1-AP | Download protocol |
| IF protocol for HP1BP3 antibody 24556-1-AP | Download protocol |
| IP protocol for HP1BP3 antibody 24556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
