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HPCAL4 Polyclonal antibody

HPCAL4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12470-1-AP

Print datasheet

Synonyms

hippocalcin like 4, Hippocalcin like protein 4, HLP4, HPCAL4

验证数据展示

  • WB analysis using 12470-1-AP

    WB analysis using 12470-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12470-1-AP (HPCAL4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human medulloblastoma using 12470-1-AP

    IHC staining of human medulloblastoma using 12470-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12470-1-AP (HPCAL4 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of fetal human brain using 12470-1-AP

    IP experiment of fetal human brain using 12470-1-AP

    IP result of anti-HPCAL4 (IP:12470-1-AP, 4ug; Detection:12470-1-AP 1:700) with fetal human brain tissue lysate 3200 ug.

经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected infetal human brain tissue
Positive IHC detected inhuman medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12470-1-AP targets HPCAL4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HPCAL4 fusion protein Ag3153 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hippocalcin like 4
Synonyms hippocalcin like 4, Hippocalcin like protein 4, HLP4, HPCAL4
Calculated Molecular Weight 191 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC030827
Gene Symbol HPCAL4
Gene ID (NCBI) 51440
RRIDAB_2120231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HPCAL4 antibody 12470-1-APDownload protocol
IHC protocol for HPCAL4 antibody 12470-1-APDownload protocol
IP protocol for HPCAL4 antibody 12470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols