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验证数据展示

  • WB analysis using 20777-1-AP

    WB analysis using 20777-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 20777-1-AP

    IHC staining of human stomach using 20777-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HPDL antibody included
  • IHC staining of human stomach using 20777-1-AP

    IHC staining of human stomach using 20777-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HPDL antibody included
  • IF Staining of Caco-2 using 20777-1-AP

    IF Staining of Caco-2 using 20777-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HPDL antibody (20777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

HPDL Polyclonal antibody

HPDL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20777-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

HPD-like protein, Glyoxalase domain-containing protein 1, GLOXD1, EC:1.13.-.-, 4-hydroxyphenylpyruvate dioxygenase-like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy HPDL Ready-To-Use IHC Kit. HPDL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20777-1-AP targets HPDL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 HPDL fusion protein Ag14773 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 4-hydroxyphenylpyruvate dioxygenase-like
别名 HPD-like protein, Glyoxalase domain-containing protein 1, GLOXD1, EC:1.13.-.-, 4-hydroxyphenylpyruvate dioxygenase-like protein
计算分子量 371 aa, 39 kDa
观测分子量 35-39 kDa
GenBank蛋白编号BC007293
基因名称 HPDL
Gene ID (NCBI) 84842
RRIDAB_10697807
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96IR7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HPDL antibody 20777-1-APDownload protocol
IHC protocol for HPDL antibody 20777-1-APDownload protocol
IF protocol for HPDL antibody 20777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Gene

Two novel heterozygous HPDL variants in a Chinese family with a neurodevelopmental disorder with progressive spasticity and brain white matter abnormalities

Authors - Yuanxuan Ma
View Article