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HPGD Monoclonal antibody, PBS Only

HPGD Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F1F5

Cat no : 66798-1-PBS

Print datasheet

Synonyms

15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1

验证数据展示

  • WB analysis using 66798-1-Ig (same clone as 66798-1-PBS)

    WB analysis using 66798-1-Ig (same clone as 66798-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66798-1-Ig (HPGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

    IF Staining of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 66798-1-Ig (HPGD antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

    IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPGD antibody included
  • IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

    IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPGD antibody included
  • FC experiment of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

    FC experiment of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human HPGD (66798-1-Ig, Clone:3F1F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HPGD Ready-To-Use IHC Kit. HPGD primary antibody included.

产品信息

66798-1-PBS targets HPGD in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity Human
Immunogen HPGD fusion protein Ag1499 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name hydroxyprostaglandin dehydrogenase 15-(NAD)
Synonyms 15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC018986
Gene Symbol HPGD
Gene ID (NCBI) 3248
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15428
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.