• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HepG2 using 66226-1-Ig (same clone as 66226-1-PBS)

    WB analysis of HepG2 using 66226-1-Ig (same clone as 66226-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66226-1-Ig (HPSE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66226-1-Ig (same clone as 66226-1-PBS)

    IHC staining of human liver cancer using 66226-1-Ig (same clone as 66226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66226-1-Ig (HPSE Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66226-1-Ig (same clone as 66226-1-PBS)

    IHC staining of human liver cancer using 66226-1-Ig (same clone as 66226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66226-1-Ig (HPSE Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66226-1-Ig (same clone as 66226-1-PBS)

    IF Staining of HepG2 using 66226-1-Ig (same clone as 66226-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66226-1-Ig(HPSE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66226-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HPSE Monoclonal antibody, PBS Only

HPSE Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66226-1-PBS

产品说明书

CloneNo. 1D8B8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Endo glucoronidase, HEP, heparanase, Heparanase 1, HPA, Hpa1, HPR1, HPSE, HPSE1, HSE1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66226-1-PBS targets HPSE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HPSE fusion protein Ag10067 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heparanase
别名 Endo glucoronidase, HEP, heparanase, Heparanase 1, HPA, Hpa1, HPR1, HPSE, HPSE1, HSE1
计算分子量 543 aa, 61 kDa
观测分子量 50 kDa
GenBank蛋白编号BC051321
基因名称 HPSE
Gene ID (NCBI) 10855
RRIDAB_2881617
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y251
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.